Relative miR-214 and PTEN protein expression levels in rat hippocampus were measured by qRT-PCR and western blot analysis.

A: Quantitative analysis of hippocampus miR-214 levels in Control, K1 and K2 group, miR-214 expression were obviously decreased in K2 group rats (n = 6; *p<0.05 compared with Control group or K1 group). B: Representative western blots of hippocampus PTEN protein levels. C: Quantitative analysis of hippocampus PTEN levels in Control, K1 and K2 group, relative PTEN protein expression levels were obviously increased in K2 group rats (n = 6; *p<0.05 compared with Control group or K1 group). GAPDH, glyceraldehyde 3-phosphate dehydrogenase.