Relative miR-214 and PTEN protein expression levels in rat hippocampus were measured by qRT-PCR and western blot analysis.

A: Quantitative analysis of hippocampus miR-214 levels in Control, K₁ and K₂ group, miR-214 expression were obviously decreased in K₂ group rats (n = 6; *p<0.05 compared with Control group or K₁ group). B: Representative western blots of hippocampus PTEN protein levels. C: Quantitative analysis of hippocampus PTEN levels in Control, K₁ and K₂ group, relative PTEN protein expression levels were obviously increased in K₂ group rats (n = 6; *p<0.05 compared with Control group or K₁ group). GAPDH, glyceraldehyde 3-phosphate dehydrogenase.