Relative fold change in differentially expressed proteins in brain tissue post-JEV infection.

Spot intensities were normalized by total valid spot intensities and mean of values from duplicate analytical gels from four biological replicates were subjected to paired t-test analysis. Protein spots showing altered expression between control and experimental groups (|ratio|≥1.5, p<0.05) were marked and excised. Dark grey bars indicate spots from mock infected control sample while light grey bars indicate JEV infected sample. * = p<0.05, # = p<0.01. Data represented are means ± SD of four independent experiments.