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Regulatory CD4⁺ CD25⁺ FoxP3⁺ T Cells Are Expanded in the Peripheral Blood of LCH Patients

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posted on 2013-02-21, 12:10 authored by Brigitte Senechal, Gaelle Elain, Eric Jeziorski, Virginie Grondin, Natacha Patey-Mariaud de Serre, Francis Jaubert, Kheira Beldjord, Arielle Lellouch, Christophe Glorion, Michel Zerah, Pierre Mary, Mohammed Barkaoui, Jean Francois Emile, Liliane Boccon-Gibod, Patrice Josset, Marianne Debré, Alain Fischer, Jean Donadieu, Frederic Geissmann

(A and B) Flow cytometry analysis of CD4⁺/CD25^hi FoxP3^high cells in the blood of LCH patients in comparison to healthy controls. (A) Representative dot plots from the blood of one patient and one control. (B) CD3 and CD4 lymphocyte counts were similar in naïve (untreated) LCH patients at diagnosis and in control children, but the absolute number and the frequency of T-regs were significantly higher in the blood of naïve LCH patients at diagnosis than in patients with LCH under treatment, in control children, and in children with SHML. *Chi-squared = 7.125 with 1 df, p = 0.0076; **Chi-squared = 13.714 with 1 df, p < 0.001; ***Chi-squared = 6.682 with 1 df, p = 0.0097.

(C) Regulatory activity of CD3⁺ CD4⁺ CD25^hi T cells isolated from the blood of LCH patients. Proliferation of control T cells (Donor T cells) induced by soluble OKT3 (Anti CD3) in the presence of CD14⁺ monocytes was inhibited by the addition of sorted CD3⁺ CD4⁺ CD25^hi T cells (LCH CD4/CD25hi) isolated by positive selection from the blood of a patient with LCH, but not by CD3⁺ CD4⁺ CD25^low T cells (LCH CD4/25lo) isolated from the blood of the same patient. *p < 0.02, **p = 0.01.