Regulation of hypothalamic COUP-TFII mRNA levels in vivo by activation of the melanocortin pathway.

(A-C) RT-qPCR analysis of mRNA levels from hypothalamus of ad libitum fed mice receiving a ICV injection of CSF (□), or 18 h fasting mice receiving a ICV injection of CSF (grey bar), or 18 h fasting mice with a ICV injection of MTII (▪). (A) BDNF mRNA levels (B) Neuro D mRNA levels (C) COUP-TFII mRNA levels. Data are expressed as mean ± SEM (n = 5) *P≤0.05 **P ≤0.01 when compared to control fed mice receiving a CSF injection. § P≤0.05 when compared to control fasting mice receiving a CSF injection. (D) Glucose and plasma insulin concentrations from the different mouse models.