Figure_6.tif (570.16 kB)
Recruitment of Epo-R into lipid rafts is dependent on Rac1 and RhoA GTPase activation.
figure
posted on 2013-02-20, 06:58 authored by Kathy L. McGraw, Gwenny M. Fuhler, Joseph O. Johnson, Justine A. Clark, Gisela C. Caceres, Lubomir Sokol, Alan F. List(A) Raft fractions were isolated from UT7 cells pretreated with 100 nM Rac1 inhibitor for 1 hr prior to Epo stimulation then immunoblotted for Epo-R with corresponding quantitation. (B) Raft fractions were isolated from UT7 cells pretreated with 100 uM ROCK inhibitor (Y-27632) for 1 h prior to Epo stimulation then immunoblotted for Epo-R with corresponding densitometry analysis. Westerns are representative of two independent experiments.
