Figure_6.tif (269.06 kB)
Radiolabeling of rCR2.
figure
posted on 2013-02-20, 19:24 authored by Adam Badar, Sarah DeFreitas, James M. McDonnell, Norhakim Yahya, David Thakor, Reza Razavi, Richard Smith, Steven Sacks, Gregory E. D. Mullen(A) rCR2 was incubated with [99mTc(CO)3(OH2)3]+ at 37°C for 30 minutes at a protein concentration of 1 µg/µL and analysed by instant thin layer chromatography (ITLC). The radiolabeling efficiency was calculated as a ratio of the radiolabeled protein peak integral (Rf = 0) to the unincorporated [99mTc(CO)3(OH2)3]+ peak integral (Rf = 1.0). (B) The radiolabeling efficiency was followed over a 120 minute period. Aliquots were removed periodically and analysed by ITLC and the radiolabeling efficiency was determined over time.
