RNAi knockdown of CSK does not affect MCF-7 cell sensitivity to tamoxifen or paclitaxel.
2013-04-04T01:25:30Z (GMT) by
<p>Cells were infected with empty lentivirus vector (pLKO.1) or two independent clones of lentiviruses expressing different shRNA species targeting CSK shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0060889#pone-0060889-g001" target="_blank">Figure 1</a> (CSK KD#1 and #2) and then exposed to 1 µM 4-hydroxytamoxifen (4-OHT) for 10 days (A) or 1–1000 nM paclitaxel for 2 days (B). Cell viability was determined by crystal violet staining (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0060889#pone.0060889.s003" target="_blank">Fig. S3</a>) and quantified by spectrophotometry (mean±SEM of three or more independent experiments).</p>