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RNA-seq sequencing yield and alignment to human (Hs19) or mouse (Mm9) genomes.

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posted on 2013-11-20, 03:42 authored by Jeffrey Chou, Matthew P. Fitzgibbon, Christie-Lynn L. Mortales, Andrea M. H. Towlerton, Melissa P. Upton, Raymond S. Yeung, Martin W. McIntosh, Edus H. Warren

Sequence reads that matched the human genome with higher fidelity (fewer mismatched bases) were classified as human reads, and those that matched the mouse genome with higher fidelity were classified as murine. Reads matching the two genomes with equal fidelity were classified as “ambiguous” and not considered further.

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