Figure_1.tif (335.88 kB)

R2d maps to a 9.3 Mb candidate interval.

figure

posted on 2015-02-13, 02:45 authored by John P. Didion, Andrew P. Morgan, Amelia M.-F. Clayshulte, Rachel C. Mcmullan, Liran Yadgary, Petko M. Petkov, Timothy A. Bell, Daniel M. Gatti, James J. Crowley, Kunjie Hua, David L. Aylor, Ling Bai, Mark Calaway, Elissa J. Chesler, John E. French, Thomas R. Geiger, Terry J. Gooch, Theodore Garland Jr., Alison H. Harrill, Kent Hunter, Leonard McMillan, Matt Holt, Darla R. Miller, Deborah A. O'Brien, Kenneth Paigen, Wenqi Pan, Lucy B. Rowe, Ginger D. Shaw, Petr SimecekPetr Simecek, Patrick F. Sullivan, Karen L Svenson, George M. Weinstock, David W. Threadgill, Daniel Pomp, Gary A. Churchill, Fernando Pardo-Manuel de Villena

CC and DO mice were crossed to generate G1 dams, which were then crossed to FVB/NJ sires to determine the TR in their progeny. Each G1 dam carries a chromosome that is recombinant for the WSB/EiJ haplotype (shown under the heading cis) and a non-WSB/EiJ chromosome (the haplotype on the homologue is shown at far right under the heading trans). Dams with the same diplotype in the central region of Chr 2 were grouped together to define ten unique diplotypes. The aggregate number of WSB/EiJ and non-WSB/EiJ alleles transmitted by dams of each diplotype are shown for dams A) with TRD and B) without TRD. Significance of TR deviation from Mendelian expectation of 0.5 was computed using one-sided binomial exact test (p-value). The contribution from the eight founders of the CC and DO are shown in different colors. Thick purple bars indicate the extent of WSB/EiJ contributions, and thin bars indicate the extent of contributions from all other strains. The black box indicates the boundaries of the R2d candidate interval as determined by the region that is WSB/EiJ in all dams with TRD.