Qualitative evaluation of the co-localization of the three different spermatogonial markers used for horses.

Considering the co-expression of GFRA1 and PLZF the following pattern was observed: A) GFRA1(+) cells (A1; red arrowhead) presenting co-localization with PLZF (A2; yellow arrowhead), as evidenced in the merged figure (A3; white arrowhead); B) this panel illustrates GFRA1(+) cells (B1; red arrowhead) that do not present PLZF expression (B2), as shown in the merged figure (B3; white arrowhead). In relation to the co-expression of GFRA1 and CSF1R the following labeling pattern was observed: C) GFRA1(+) cells (C1; red arrowhead) also expressing CSF1R (C2; green arrowhead), shown in the merged figure (C3; white arrowhead); D) differently, some GFRA1(+) cells (D1; red arrowhead) do not present CSF1R (D2; white arrowhead in D3 merged figure). E) Summarization of the quantitative data obtained for Aund GFRA1, PLZF and CSF1R positive cells in horses, suggesting that these three proteins are differently expressed in this cell population. Yellow bar = 20 µm; White bar = 30 µm.