Fig_7.tif (584.68 kB)
Protein recovery analysis in the process of optimized-DS, ProteoMiner and gel-filter approaches for human serum samples.
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posted on 2015-02-27, 03:57 authored by Lingsheng Chen, Linhui Zhai, Yanchang Li, Ning Li, Chengpu Zhang, Lingyan Ping, Lei Chang, Junzhu Wu, Xiangping Li, Deshun Shi, Ping XuA. Peptide quantification with SILAC labeled GST as internal standard. Same amount of peptides digested from heavy labeled GST was spiked in the samples processed with optimized-DS, ProteoMiner and gel-filter methods, and then analyzed by LC-MS/MS. B. Histogram of protein recovery after the treatment through these three strategies. Error bars indicate mean ± SD. The whole experiment was repeated twice (*: P < 0.05, **: P < 0.01).
