Protein expression profile and differentiation of the primary and 5<sup>th</sup> passaged BM-MSCs toward cardiocytes and vascular endothelial cells <i>in vitro</i>.

<p>(A) The representative images of silver stained 2-DE gel of the proteins from the primary pour-off cultures (upper panel, 1<sup>st</sup> BM-MSCs) and the 5<sup>th</sup> passaged cultures (low panel, 5<sup>th</sup> BM-MSCs). (B) Desmin, (C) Troponin I, (D) MHC-ß, (E) VEGF, (F) FLT-1 and (G) KDR mRNA levels were examined in BM-MSCs from the primary pour-off cultures (1<sup>st</sup> BM-MSCs) and the 5<sup>th</sup> passaged cultures (5<sup>th</sup> BM-MSCs) induced by 5′-azacytidine (B–D) and VEGF (E–G) by real-time RT-PCR. The mRNA levels were calculated as a ratio to the GAPDH mRNA level and expressed relative to levels of the 1<sup>st</sup> BM-MSCs. Each value was the means±S.E.M. of 5 determinations. **: P<0.01; ***: P<0.001 compared with the 1<sup>st</sup> BM-MSCs.</p>