Proportions of sporocyte and mature cyst stages of <i>Pneumocystis carinii</i>.

<p>Whole organism population was isolated from the lungs of immunosuppressed animals as previously described <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0020935#pone.0020935-Aviles1" target="_blank">[27]</a>. Sporocyte and mature cyst stages were counted from RAL-555-stained smears according to the number of nuclei in each life cycle stage. Percentages of sporocyte and cystic stages with N nuclei in the fraction of sporocytes and mature cysts were given as mean of three independent experiments (n = 70, n = 55, n = 50). N = number of nuclei per fungal cell. SD = standard deviation.</p>(a)<p>Early sporocytes.</p>(b)<p>Sum of intermediate sporocytes with 2, 3 and 4 nuclei.</p>(c)<p>Sum of late sporocytes and mature cysts with 5, 6, 7 and 8 nuclei.</p>

License

CC BY 4.0