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Progenitor cell evaluation by flow cytometry.

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posted on 2013-09-12, 02:08 authored by Lucia Cuppini, Angelica Calleri, Maria Grazia Bruzzone, Elena Prodi, Elena Anghileri, Serena Pellegatta, Patrizia Mancuso, Paola Porrati, Anna Luisa Di Stefano, Mauro Ceroni, Francesco Bertolini, Gaetano Finocchiaro, Marica Eoli

A: Gate used to exclude cell fragments and debris. B: Gate made to include CD45- and CD45dim cells. C: Gate on Syto16+ 7AAD+ cells. D: Identification of 2 different populations: CD45-CD34+ + and CD133-VEGFR2- (D1), and CD45dimCD34+ and CD133+ cells (D2).

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