Figure_7.tif (473.25 kB)
Profiles of density of HCV produced in different hepatoma cell lines.
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posted on 2013-08-05, 04:51 authored by Francois Helle, Etienne Brochot, Carole Fournier, Véronique Descamps, Laure Izquierdo, Thomas W. Hoffmann, Virginie Morel, Yves-Edouard Herpe, Abderrahmane Bengrine, Sandrine Belouzard, Czeslaw Wychowski, Jean Dubuisson, Catherine Francois, Jean-Marc Regimbeau, Sandrine Castelain, Gilles DuverlieHuH-7 (A), Hep3B (B), HepG2-CD81 (C) and PLC/PRF/5 (D) were electroporated with in vitro transcribed RNA of the JFH1-CS-A4-RLuc genome containing mutations R1373Q/C2441S. The supernatants of each electroporated cell lines were recovered six days post-electroporation and overlaid on 10 to 50% (weight/volume) iodixanol gradients. After a 24 h ultracentrifugation, sixteen fractions were collected and analyzed for HCV RNA quantity and infectivity on naive HuH-7 cells (assessed by measuring Renilla Luciferase activities). The results are expressed as percentages of total infectivity or HCV RNA and are reported as means of two independent experiments.
