Table_5.xls (9.5 kB)
Primers and probe used for bucl amplificationa.
dataset
posted on 2015-09-10, 03:45 authored by Beth A. Bachert, Soo J. Choi, Anna K. Snyder, Rita V. M. Rio, Brandon C. Durney, Lisa A. Holland, Kei Amemiya, Susan L. Welkos, Joel A. Bozue, Christopher K. Cote, Rita Berisio, Slawomir Lukomskia Primers were designed to generate conserved amplicons within the non-collagenous region of each bucl gene. Primers for bucl6 and bucl15 were designed flanking the collagenous region, which varies in size among strains (S4B Fig). Primers Bucl8_3F/3R were used to generate amplicon from Bp K96243 reference strain, whereas primers Bucl8_2F/2R selectively amplify products from Bp and Bm gDNA and were used in large-scale PCR screening (S4B Fig).
b Amplicon sizes expected for the Bp K96243 reference strain.
c LNA probe for bucl16 detection; + symbols precede LNA bases.
Primers and probe used for bucl amplificationa.
