Figure_5.tif (3.42 MB)
Pre-embedding immunoelectron microscopic analysis shows the presence of Mieap and lysosomal proteins within mitochondria.
figure
posted on 2011-01-17, 01:58 authored by Yuji Miyamoto, Noriaki Kitamura, Yasuyuki Nakamura, Manabu Futamura, Takafumi Miyamoto, Masaki Yoshida, Masaya Ono, Shizuko Ichinose, Hirofumi ArakawaThe Ad-Mieap and Ad-LacZ infected cells of HCT116 were subjected to pre-embedding imunoelectron microscopic analysis with DAB on day 3 after IR. The primary antibody against Mieap (A), cathepsin D (B), or LAMP1 (C) was used for the analysis. The representative images are shown. Arrowheads (yellow) indicate representative mitochondria. N: nucleus CL: cytoplasmic lysosome Scale bar = 2 µm.
