Phylogenetic tree of the MLSA based on six concatenated housekeeping genes of the genus Thalassospira.

The tree was constructed using the neighbor-joining method with MEGA 5.0. Bootstrap values over 50% (1000 replications) were shown at each node. Bar, % estimated substitution. Rhodospirillum rubrum ATCC 11170^T (NC_007643) was used as the outgroup. Meantime, the isolated areas and water depth of the Thalassospira bacteria were divided artificially and marked by different symbols or colors.