Figure_2.tif (2.56 MB)
Phenotypic analysis of glxA mutant.
figure
posted on 2013-01-11, 02:43 authored by Recep Liman, Paul D. Facey, Geertje van Keulen, Paul J. Dyson, Ricardo Del SolS. coelicolor M145, cslA− (cslA-Tn) and glxA− strains are indistinguishable when grown on MS agar (A) but both mutant strains display a bld phenotype on R5 (B). The bld phenotype in mutants cslA− and glxA− cannot be rescued by extracellular complementation by the parental strain S. coelicolor M145 (C). The bld phenotype of glxA− is conditional to the presence of high osmolyte in the medium. Strains shown were grown on R5 without Sucrose for 5 days (D).