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Phenotypic analysis of glxA mutant.

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posted on 2013-01-11, 02:43 authored by Recep Liman, Paul D. Facey, Geertje van Keulen, Paul J. Dyson, Ricardo Del Sol

S. coelicolor M145, cslA (cslA-Tn) and glxA strains are indistinguishable when grown on MS agar (A) but both mutant strains display a bld phenotype on R5 (B). The bld phenotype in mutants cslA and glxA cannot be rescued by extracellular complementation by the parental strain S. coelicolor M145 (C). The bld phenotype of glxA is conditional to the presence of high osmolyte in the medium. Strains shown were grown on R5 without Sucrose for 5 days (D).

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