Phenazine synthesis by P. aeruginosa is essential for killing C. elegans.

(A) Phenazine synthesis pathway of P. aeruginosa. (B) Killing of wild-type P. aeruginosa PA14 and Δphz mutant and partial complementation of Δphz killing with plasmids containing either the phzA1-G1 or phzA2-G2 operon. (C) Complementation of killing in the Δphz mutant by addition of synthetic phenazine-1-carboxylic acid (100 µg/mL) to the agar medium prior to plating and growth of the bacteria.