Persistence of L. pneumophila in two-species biofilms formed by K. pneumoniae and Flavobacterium sp. or K. pneumoniae and P. aeruginosa.

A base biofilm of either K. pneumoniae strain DMDS 92-08-28a (□) and Flavobacterium sp. strain CDC-65 (◊) (A) or K. pneumoniae strain DMDS 92-08-28a (□) and P. aeruginosa ATCC strain 7700 (◊) (B) pre-formed on stainless steel coupons was inoculated with L. pneumophila strain 130b (▪) on day 3 and flow of 1∶100 R2A began at 1–2 ml/min. Each data point represents the averages and standard deviations of colony counts from a single rod, and the experiments shown here are representative of two experiments.