Performance comparison of the recovery rater for LMW proteins from human serum using optimized-DS, ProteoMiner and gel-filter methods.

A. Human serum proteome analyzed by the optimized-DS approach. On lane 1, 2 μL of untreated human serum was loaded. On lane 3, 10 μL of human serum was processed with the optimized-DS method after mixed with 0.3 μg regular GST protein. B. The human serum was processed with ProteoMiner method. On lane2, 2 μL of untreated human serum was saved as control as in A. On lane 3 (a) and 4 (b), 5% of eluates from the starting 200 μL of human serum sample treated with ProteoMiner was mixed with 0.1 or 0.3 μg of regular GST proteins and resolved on SDS-PAGE gel. C. Gel-filter analysis of human serum. On lane 2–4 (a-c), 10 μL of human serum samples were mixed with 0.3 μg of regular GST protein and analyzed by gel-filter method. On lane 4 (A) and 5 (B and C), 0.3 μg of regular GST protein alone was loaded as control.