Percentage of aberrant marker segregation in msh4, msh5 mutants in the NHY942/NHY943 strain background.

Non 2∶2 segregation of markers in msh4 and msh5 mutants were identified from four spore viable tetrads using RANA software [24]. All aberrant segregants were 1∶3 or 3∶1 gene conversions except for two 4∶0 events. No post-meiotic segregation events were observed. Gene conversion data for wild-type, pch2Δ, spo11-HA and spo11-HA pch2Δ are from Zanders and Alani [21].