Partial loss of <i>Fer2</i> expression impairs the viability of PAM neurons in aging flies.

<p>Quantification of PAM neurons in the flies of different genotypes detected by anti-TH staining at indicated ages. Box boundaries are the 25th and 75th percentiles, the horizontal line across the box is the median, and the whiskers are the lowest and highest values that are not outliers. The open circles represent outliers and closed circles are extreme cases. *p<0.05, **p<0.01, *** p<0.001, comparing <i>Fer2<sup>2</sup>/+</i> and <i>Fer2<sup>2</sup></i> at the same age (A), or between <i>miR-Fer2-N</i> and <i>miR-Fer2-4/</i>-<i>5</i> at the same age (B-D). Sample sizes are indicated in the <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004718#s4" target="_blank">Materials and Methods</a>. (A) PAM neurons in <i>Fer2<sup>2</sup></i> and the control (<i>Fer2<sup>2</sup>/+</i>). (B) Constitutive expression of <i>miR Fer2s</i> by <i>Fer2-GAL4.</i> (C) Constitutive expression of <i>miR Fer2s</i> by <i>R58E02-GAL4</i>. (D) Adult-specific <i>Fer2</i> knockdown and the control. <i>Fer2-GAL4</i>, <i>tub-gal80<sup>ts</sup></i>: control without UAS-transgene.</p>