Paralysis assay in a transgenic C. elegans (CL4176) expressing Aβ.

Age synchronized CL4176 worms were treated from egg until the L3 larval stage with CIN, GS (10 µg/ml) and SQDB (100 µg/ml). The animals were allowed to cultivate at 16°C for 38 h. At the 38 h time point, the temperature was up-shifted to 23°C. Worms began to paralyze 25 h after the temperature up-shift and were scored at 1 hr intervals. Data are expressed as percent (%) of worms not paralyzed. SQDB, GS and CIN alleviate β amyloid-induced toxicity in the transgenic C. elegans strain.