Pak1, Pak2 and Pak3 isoforms were detected in WT murine tracheal smooth muscle by immunoblot.

<p>No Pak1 was detected in extracts of isolated tracheal smooth muscle (A) or whole tracheas (B) from <i>Pak1</i><sup>−/−</sup> mice. Immunoblots of tracheal smooth muscle were obtained from tracheal smooth muscle extracts pooled from 3 mice of each type. Whole trachea extracts were each from a single mouse.</p>

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