Figure_2.tif (1.73 MB)
P. ovale rbp2 qPCR dynamic range.
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posted on 2015-01-15, 02:58 authored by Robin H. Miller, Clifford O. Obuya, Elizabeth W. Wanja, Bernhards Ogutu, John Waitumbi, Shirley Luckhart, V. Ann StewartA ten-fold serial dilution of rbp2 plasmid (1 to 100,000 copies/μl) is shown in the amplification plot. The cycle threshold was determined automatically by the ABI 7500 fast system software program. The negative control sample (red line) shows no amplification over the cycle threshold for 60 cycles.
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Western Kenyaovale rbp 2 qPCR assaymalaria burden.MethodsIn orderPlasmodium ovale curtisiovale subspeciesovale wallikeri.ResultsOur Pmultilocus genotypingmalaria parasite speciesovale wallikeriovale curtisirbp 2 qPCR assayreticulocyte binding protein 2ovale subspecies prevalenceWestern Kenya Utilizingdnaovale rbp 2 qPCR assay validation experimentsrnarbp 2 gene.ConclusionsOur novel Platency duration differences
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