PI3-K is required for resveratrol-inhibited the phosphorylation of AMPK and the expression of the SIRT1 protein induced by LPS in RAW 264.7 macrophage cells.

RAW 264.7 cells were pre-treated with resveratrol (5 µM) for 1 h in the absence or presence of wortmannin (1 µM), then exposed to LPS (5 µg/ml) for 40 min (Pane A) and 6 h (Panel B). Cell lysates (50 µg protein) were prepared and subjected to Western blot analysis by using antibodies specific for total AMPK, phosphorylated forms of AMPK (shown as phospho-AMPK), Sirt1 and β-actin as described in the methods. The values shown are mean ± SEM of data from three independent experiments. ^#Significant compared with control alone, p<0.05. *Significant compared with LPS alone, p<0.05. ^ΔSignificant compared with resveratrol + LPS, p<0.05.