PI3K does not regulate the LIF-dependent plasticity window but stimulates differentiation towards early mesoderm lineage while inhibiting ectoderm and endoderm differentiation processes.

<p>Cells were depleted from LIF or induced with LIF in the presence of LY, for the first 24 hours as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0146281#pone.0146281.g001" target="_blank">Fig 1C</a>. After 5 days, expression of the selected genes (A) <i>Master</i> genes, (B) <i>Pluri</i> genes, (C) early <i>Diff</i> genes and (D) specific germ layer genes, was analyzed by RT-qPCR. Graphs represent the average level of expression and SEM from 4 independent experiments. Paired sample t-test was performed to evaluate the significance of the difference in the expression levels observed with or without LY: *p-value<0.05; **p-value<0.01, ***p-value<0.001; a: pvalue = 0.051; if not stated: not significant.</p>