PDF-1 secretion is not altered in <i>glr-2</i> mutants.

<p>PDF-1 secretion was analyzed in the indicated genotypes. YFP-tagged PDF-1 was expressed with the <i>pdf-1</i> promoter. Representative images (A) and summary data (cumulative fraction) (B-C) are shown for coelomocyte fluorescence in L4/A lethargus and 1-day old adults of the indicated genotypes. PDF-1::YFP coelomocyte fluorescence was dramatically increased in <i>npr-1</i> mutants during the L4/A lethargus and in adults as previously reported [<a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005359#pgen.1005359.ref011" target="_blank">11</a>]. Mutations inactivating GLR-2 did not alter PDF-1::YFP coelomocyte fluorescence during L4/A lethargus (B) and in adults (C) in either wild type or <i>npr-1</i> mutants. Scale bar indicates 10 μm. <i>p</i> values are indicated for each comparison (Kolmogorov-Smirnov test).</p>