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PCR analysis of 3 V. albo-atrum transformants obtained from ATMT experiments on potato.

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posted on 2013-02-20, 22:44 authored by Claire J. Knight, Andy M. Bailey, Gary D. Foster

Lane 1, DNA Hyperladder I (Bioline), lanes 2–7, PCR with DsRed primers; 3 transformants (2–4), wild type V. albo-atrum (5), pCAMDsRed (6), water (7). Lanes 8–13, PCR with hygromycin B primers; 3 transformants (8–10), wild type V. albo-atrum (11), pCAMDsRed (12), water (13). Lanes 14–19, PCR with wild type V. albo-atrum ITS primers; 3 transformants (14–16), wild type V. albo-atrum (17), pCAMDsRed (18), water (19).

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