Oxidation-induced denaturation of proteins in Lgr4 knockout lenses.

Lens supernatants of 10 weeks (10 W) and >18 weeks (>18 W) diluted to 1 mg/mL protein using the TC-199 medium were subjected to oxidative damage with 0.1 mM H₂O₂ and 2.31 units of glucose oxidase (GO) for indicated times. Sample absorbance was measured at 360 nM. Supernatants from Lgr4 knockout lenses showed a more rapid increase in turbidity (and therefore, protein denaturation), suggesting that the knockout lens had less overall resistance to oxidation than wild-type. Data are expressed as mean ± SD (n = 3). These results are representative of 3 independent experiments.