Oxidated/nitrated wild-type and mutant p53 in EOSAD, ADmut, and control lymphocytes.

Representative blot of data obtained from PS1P117R mut (n = 1), EOSAD (n = 1) and control (n = 1) lymphocytes. Equal amount of protein from control, ADmut and EOSAD lymphocytes were immunoprecipitated with conformation specific anti-p53 antibodies, and immunoprecipitates were analyzed for HNE (A) or 3NT (B) immunoreactivity by Western blotting. (C) Graphical analysis of HNE-p53 band intensities of controls (n = 5), EOSAD (n = 5), and ADmut (n = 6). p53 expression was used to normalize the intensity of each band. Bars represent median value of the respective group. Data are expressed as mean ± SEM. (D) Graphical analysis of 3NT-p53 band intensities of controls (n = 5), EOSAD (n = 5) and ADmut (n = 6). p53 expression was used to normalize the intensity of each band. Bars represent median value of the respective group. Data are expressed as mean ± SEM. * p<0,01.