Ox-LDL loading induces the secretion of visfatin and resistin in adipocytes.

Differentiated 3T3-L1 adipocytes were incubated in medium containing various concentration of ox-LDL (0, 25, 50， 75， 100µg/ml) and 10µg/ml Sandoz 58035 for 48hrs(A); Adipocytes were incubated with ox-LDL (50µg/ml) in a combination of sandoz58035(10µg/ml) for various times(B). At the end of the incubation, the mRNA expressions of visfatin and resistin were evaluated by real-time PCR. The culture media were collected, and the amounts of visfatin and resistin released to the media were analyzed by enzyme-linked immunosorbent assay. * P<0.05, as compared with control; # P<0.01, as compared with control. Data are presented as means ± SD (n = 3).