Overview of the RT-qPCR based method for immunodiagnosis of <i>M. tuberculosis</i> infection.

<p>A. Blood collection and incubation at 37C. B. Extraction of RNA. C. One-step probe based RT-qPCR and calculation of IP-10 mRNA upregulation using ΔΔCt. D. Optional RNA storage on dried blood spots for easy sample transportation.</p>