Figure_1.tif (1.26 MB)
Overview of the RT-qPCR based method for immunodiagnosis of M. tuberculosis infection.
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posted on 2014-09-03, 03:23 authored by Thomas Blauenfeldt, Jan Heyckendorf, Sidse Graff Jensen, Christoph Lange, Camilla Drabe, Thomas S. Hermansen, Lena de Thurah, Troels Lillebaek, Jesper Eugen-Olsen, Niels Seersholm, Søren Hoff, Jesper Bonde, Morten RuhwaldA. Blood collection and incubation at 37C. B. Extraction of RNA. C. One-step probe based RT-qPCR and calculation of IP-10 mRNA upregulation using ΔΔCt. D. Optional RNA storage on dried blood spots for easy sample transportation.
