Overexpression of miR-217 inhibits the osteosarcoma cell line MG-63 proliferation, migration, and invasion.

<p>(A) Expression levels of miR-217 were examined by qRT-PCR after transfection of 20 nmol/L of miR-217 mimics, inhibitors or sramble or no transfection in the cell line MG-63. (B) The cells treated with miR-217 mimics, inhibitors or sramble or no transfection were measured by CCK8 assay at different time periods. (C) Wound healing assays of MG-63cells after treatment with miRNA mimics, inhibitors or scramble or no transfection; the relative ratio of wound closure per field is shown. (D) Transwell analysis of MG-63 cells after treatment withmiRNA mimics, inhibitors or scramble or no transfection; the invasive cells per field is shown below, All data uses t test and is shown as mean ±SEM. *p<0.05,** p<0.01, and ***p<0.001.</p>