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Overexpression of human AGGF1 in a mouse hindlimb ischemia model does not affect the expression level of VEGF.

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posted on 2013-02-19, 21:29 authored by Qiulun Lu, Yihong Yao, Yufeng Yao, Shizhi Liu, Yuan Huang, Shan Lu, Ying Bai, Bisheng Zhou, Yan Xu, Lei Li, Nan Wang, Li Wang, Jie Zhang, Xiang Cheng, Gangjian Qin, Wei Ma, Chengqi Xu, Xin Tu, Qing Wang

Seven days after delivery of AGGF1 expression plasmid DNA (empty vector as control), mice were sacrificed and gastrocnemius muscle tissues were excised and used for isolation of total RNA and follow-up real time RT-PCR analysis of the mouse VEGF gene. Note that VEGF expression did not show any difference between AGGF1 and control (P>0.05).

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