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Overexpression of corp+ suppresses the BARTL phenotype.

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posted on 2015-07-31, 03:16 authored by Riddhita Chakraborty, Ying Li, Lei Zhou, Kent G. Golic

(A) The range of eye phenotypes observed in the assay. The BS phenotype of H1 control males is shown at the left. When FLP is expressed (ey>FLP), the phenotypes can range from headless pharates (category 1) to adults with a fully developed wildtype eye (category 5). The distribution produced (B) in control males; (C) in males carrying P{EPgy2}EY03495, referred to as EP-corp+; (D) by inclusion of a UAS-corp+ transgene; (E) with RNAi-mediated knockdown of corp; (F) in corp95B mutants; (G) with rescue of the corp95B phenotype by expression from the UAS-corp+ transgene; and, (H) with RNAi-mediated knockdown of CG1632. N represents number of eyes scored for each genotype. Each P value represents comparison with the wildtype control, shown in B. Genotypes were: (B) y w/H1; eyGal4 UAS-FLP/+; (C) y w EP-corp+/H1; eyGal4 UAS-FLP/+; (D) y w/H1; eyGal4 UAS-FLP/+; UAS-corp+/+; (E) y w/H1; eyGal4 UAS-FLP/RNAi-corp; (F) y w corp95B/H1; eyGal4 UAS-FLP/+; (G) y w corp95B/H1; eyGal4 UAS-FLP/+; UAS-corp+/+; (H) y w/H1; eyGal4 UAS-FLP/RNAi-CG1632.

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