Overexpression of MGAT3 inhibits the migration ability of epithelial ovarian cancer cells.

SKOV3-ip cells were transfected with pcDNA3.1 empty or MGAT3/pcDNA3.1 vectors. At 48 h after transfection, amounts of MGAT3 protein and its catalysate, N-glycans containing bisecting GlcNAc were detected by Western blotting (A) and Lectin blotting (B) in SKOV3-ip cells, respectively. Human beta-actin served as an endogenous control. All the relative expression levels of N-glycans containing bisecting GlcNAc were normalized to beta-actin. The left column of (B) represents the densitometry result for glycoproteins 50–250 kDa relative to control set at 1.0. It should be noted that the expression levels of MAGT3 and its catalysate, bisecting glycans were both significantly increased. Simultaneously, cells were subjected to the migration assay. Images of migrating cells from migration assay (C) and quantitative results are shown (D). Original magnification: 200x.The reported values are expressed as the means ± SEM. Each assay was performed at least three times. A p-value of less than 0.05 indicates statistical significance using Student’s t-test.