Over-expressed MMP-1 influences gastric cancer cell invasion and schematic model of galectin-3 regulate gastric cancer cell invasion and migration.

A, protein expression of galectin-3, PAR-1, MMP-1, phospho-Paxillin (pY181) and paxillin levels were measured by western blot analysis, after infection with lenti-viral construct containing pLECE3 (vector only) and pLECE3-MMP-1 in AGS cells, transfected with galectin-3 or PAR-1 siRNAs. B, Cell invasion performed by the above cells present as a histogram (* p<0.001 vs. Cont group). C, Galecitn-3 enhances PAR-1 expression via binding with AP-1 transcription factor, also, galectin-3 regulation of MMP-1 expression. MMP-1 increase causes dual effects in gastric cancer invasion; 1) cleavage of PAR-1 tethered ligand and PAR-1 activation 2) degradation of extracellular matrices (ECM).