Table_1.xls (9.5 kB)
Oligonucleotides used for Luminex MOL-PCR.
dataset
posted on 2012-07-20, 01:47 authored by David Stucki, Bijaya Malla, Simon Hostettler, Thembela Huna, Julia Feldmann, Dorothy Yeboah-Manu, Sonia Borrell, Lukas Fenner, Iñaki Comas, Mireia Coscollà, Sebastien Gagneux1
Nomenclature according to [2].
2Position of SNP in reference to H37Rv.
3SNP in an essential gene (“ess”); synonymous SNP (“syn”).
4Strand orientation of oligonucleotides.
5LPO, left probe oligonucleotide, annealing upstream of SNP of interest and including it; RPO, right probe oligonucleotide, annealing downstream of SNP of interest.
6xTag bead with coupled antiTag-sequence.
7Universal primer sequences (upper case, not underlined), allele-specific tag-sequences (lower case), and sequences hybridizing to the template genomic sequence (underlined). RPO are 5′-phosphorylated.
