OMV-bound Pla is catalytically active and interacts with components of the ECM.

<p>(A) Plg-activating ability of wild-type or Δ<i>pla Y. pestis</i> bacteria or OMVs. Whole bacteria or purified OMVs were incubated with human glu-plg and a fluorescent substrate of plasmin for 3 hours at 37°C. (B) Degradation of α2-antiplasmin by wild-type or Δ<i>pla Y. pestis</i> bacteria or OMVs. Whole bacteria or purified OMVs were incubated with purified human α2-antiplasmin at 37°C and at the times indicated, the presence of uncleaved α2-antiplasmin was determined by immunoblot analysis.</p>