Figure_5.tif (676.46 kB)
OMV-bound Pla is catalytically active and interacts with components of the ECM.
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posted on 2014-09-08, 03:16 authored by Justin L. Eddy, Lindsay M. Gielda, Adam J. Caulfield, Stephanie M. Rangel, Wyndham W. Lathem(A) Plg-activating ability of wild-type or Δpla Y. pestis bacteria or OMVs. Whole bacteria or purified OMVs were incubated with human glu-plg and a fluorescent substrate of plasmin for 3 hours at 37°C. (B) Degradation of α2-antiplasmin by wild-type or Δpla Y. pestis bacteria or OMVs. Whole bacteria or purified OMVs were incubated with purified human α2-antiplasmin at 37°C and at the times indicated, the presence of uncleaved α2-antiplasmin was determined by immunoblot analysis.
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protease PlaYersinia pestismembrane vesiclesplasminogen activationfimbrial antigenBraun lipoproteinMembrane proteinsPlague Pathogen Yersinia pestisVirulence factorsPla substratesFas ligandF 1membrane stressdisease plaguepestis OMVsinfectionOMV releaseadhesin AilOuter Membrane VesiclesCell growthExtracellular matrix100 nm
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