Notch pathway inhibition on BM-PC reduces their pro-angiogenic properties <i>in vitro</i>.

<p>A. Quantitative analysis of Matrigel-induced tube branching of HUVEC untreated or GSI-treated monoculture and co-cultured with control and GSI-treated BM-PC. Results show the average number of branch points in 5 high power fields. B. Representative images of HUVEC tube formation in monoculture and co-cultured with control and GSI treated BM-PC. Phase contrast microscopy (original magnification, 40×). C. Quantitative analysis of Matrigel-induced tube branching of HUVEC untreated or GSI-treated monoculture and co-cultured with control and GSI-treated BM-PC, using different doses of GSI treatment. D. Representative images of HUVEC tube structures in the presence of control or GSI BM-PC acetylated LDL-FITC labelled (original magnification, 40×). Arrows identify acetylated LDL-FITC labelled control or GSI BM-PC. E. Quantification of control or GSI BM-PC found in or out of endothelial tubular structures. Results expressed relatively to the total number of BM-PC counted. *P<0.05. Each experiment was performed in triplicate and the mean presented (n = 3).</p>