Neural stem cells (NSCs) derived from human embryonic stem cells H9 and hiPS maintain differentiation potential.

A. NESTIN staining indicates that close to 100% positive NSCs are derived from H9 and hiPS. B. qPCR showed that hESCs (H9) and iPS highly express pluripotency markers Oct4, Nanog and Sox2, yet NSCs highly express NSCs markers Pax6 and Nestin. C. H9 and hiPS derived NSCs can differentiate into both neural and glial lineage as stained by neuron marker TUJ-1, astrocyte marker GFAP and oligodendrocyte marker Olig2. D. H9 and E. iPS derived NSCs differentiated in time-dependent manner, with predicated gene expression pattern. w, abbr. of week.