NPcis cell lines do not produce any functional Type-I IFN in vitro nor does MYXV treatment produce a Type-I IFN response in vivo.

A – Type-I interferon production in NPcis cell lines in response to 1.0MOI MYXV-GFP (MYXV), 1.0 MOI of oncolytic vesicular virus (VSV-Δ51), 25 ug/uL delivered extracellulary (pI∶C EC), 1.0 µg/uL polyI∶C delivered by intracellularly via lipofectimine (pI∶C IV), UV-inactivated MYXV (DV) or no treatment (CT) as measured using the HEK-Blue system 24 hpi. Error bars represent standard error and asterisks p<0.05 compared to control. B – Reverse-transcriptase (RT)-PCR of Type-I interferons and Type-I interferon responsive genes in K1492 and K1861 lines in response to 1.0 MOI vMyx-GFP (MYXV), 1.0 MOI of oncolytic vesicular virus (VSV), 1.0 µg/uL intracellular polyI∶C (pI∶C), 10 Units of exogenous mouse IFNβ (IFNβ), UV-inactivated MYXV (DV) or no treatment (CT). C - Elisa of mouse IFNβ and mouse pan IFNα from 14 day K1492 tumours 1, 3 or 7 days post intracranial administration of 5×10⁶ PFU vMyx-FLuc (MYXV), UV-inactivated MYXV (DV) or 5 µg of naked polyI∶C (pI∶C), n = 2 per time point. Stippled bar represents K1492 tumour at 14, 15, 17, and 21 days with combined with no treatment (n = 2 per time point). Left-slashed bars are normal mouse brain (n = 2) and right-slashed bars are normal mouse brain spiked with 15 pg and 20 pg of IFNβ or IFNα, respectively. Error bars represent standard error and asterisks p<0.05 compared to normal brain.