Figure_1.tif (486.46 kB)
Mycolactone is cytotoxic for keratinocytes.
figure
posted on 2013-02-20, 22:29 authored by Alvar Grönberg, Louise Zettergren, Kerstin Bergh, Mona Ståhle, Johan Heilborn, Kristian Ängeby, Pamela L. Small, Hannah Akuffo, Sven BrittonMycolactone was added to sub-confluent cultures of keratinocytes and (A) cell numbers were measured as optical density of WST-1after 24, 48 and 72 h of treatment with 1000 ng/ml of mycolactone, or (B) after 72 h at different concentrations of mycolactone. An asterisk indicates a significant (p<0.05) reduction in WST-1 labeling as compared to the corresponding medium control. Cell cultures of untreated controls (C) and cultures treated with 300 ng/m mycolactone (D) were photographed after 48 h. Data are from one representative experiment showing means and SD of triplicates.