Mutations in the NR4A2 LBD result in mis-localisation of the receptor with DNA repair proteins.

(A) Schematic representation of the WT and ΔH12 mutant forms of NR4A2 indicating the position of the C-terminal helix 12. MM96L cells transfected with WT or ΔH12 forms of NR4A2 were treated with 25 mJ/cm2 UV, fixed at 4 hours post-UV treatment. Immunofluorescence was performed to detect (B) γH2A.X; (C) DDB2; and (D) XPC proteins. Left panels (green fluorescence), pEYFP-NR4A-WT (upper) or pEYFP-NR4A2-ΔH12 (lower); Middle panels, γH2A.X, DDB2 or XPC immunofluorescence (red); Right panels, merged images; Representative images from 4 independent experiments is shown.