Multivariate discriminant analysis of the <i>UBE2D2</i> normalised gene GeXP hCellMarkerPlex data from human colon normal (white triangle) (n = 24), adenomatous polyp (grey triangle) (n = 17) and carcinoma (black triangle) (n = 19) tissues.

<p>Information on the gene symbols on the biplot is available in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0113071#pone.0113071.s002" target="_blank">Table S2</a>. (A) Principal component analysis (PCA) biplot permits visualisation of inherent clustering patterns of individual tissue samples and associated gene expression levels. (B) Orthogonal Partial Least Squares Discriminant Analysis (OPLS-DA) was applied to fit a 2-class supervised model maximising covariance and discriminating gene expression profiles associated with the different tissues sample types; the biplot shows scores and loadings as well as the regression coefficients best explaining each class ($M4.DA(N),$M4.DA(A),$M4.DA(C). (C) Rank of importance of cell marker genes within the OPLS-DA. (D) Matrix showing the associated misclassification rates.</p>