Multiple sequence alignment of predicted soybean HSP90 protein.

Multiple-aligned sequences were determined by ClustalX, and GeneDoc was used to manually edit the results. Identical or similar acids are shown in black or gray, respectively. The HATpase_c signature sequence (red line) and signature motif ‘Y-x-[NQHD]-[KHR]-[DE]-[IVA]-F-[LM]-R-[ED]’ (yellow rectangle drawn) of Hsp90 were detected by scanning sequences against PROSITE patterns and profiles at http://web.expasy.org. The motif MEEVD, KDEL, DPW which is diagnostic of cytoplasmic, ER-retention, chloroplast and mitochondrial Hsp90, respectively, was bounded by a green rectangle drawn.