Multiple-sequence alignment of ABCA4 from different species.

Multiple-sequence alignment in ABCA4 from different species revealed that these mutations were located within a highly conserved region. The mutations were highlighted by red box. Majority of the identified mutations (4 of 6) were located within the alpha-helix regions. α-helices are displayed as squiggles; β-strands as arrows; strict β-turns as TT letters.